Evaluation of various cleaning methods to remove bacillus spores from spacecraft hardware materials

A detailed study was made of the biological cleaning effectiveness, defined in terms of the ability to remove bacterial spores, of a number of methods used to clean hardware surfaces. Aluminum (Al 6061) and titanium (Ti 6Al-4V) were chosen for the study as they were deemed the two materials most likely to be used in spacecraft extraterrestrial sampler construction. Metal coupons (1 cm x 2.5 cm) were precleaned and inoculated with 5.8 x 10(3) cultivable Bacillus subtilis spores, which are commonly found on spacecraft surfaces and in the assembly environments. The inoculated coupons were subsequently cleaned using: (1) 70% isopropyl alcohol wipe; (2) water wipe; (3) multiple-solvent flight-hardware cleaning procedures used at the Jet Propulsion Laboratory (JPL); (4) Johnson Space Center-developed ultrapure water rinse; and (5) a commercial, semi-aqueous, multiple-solvent (SAMS) cleaning process. The biological cleaning effectiveness was measured by agar plate assay, sterility test (growing in liquid media), and epifluorescent microscopy. None of the cleaning protocols tested completely removed viable spores from the surface of the aluminum. In contrast, titanium was capable of being cleaned to sterility by two methods, the JPL standard and the commercial SAMS cleaning process. Further investigation showed that the passivation step employed in the JPL standard method is an effective surface sterilant on both metals but not compatible with aluminum. It is recommended that titanium (Ti 6Al-4V) be considered superior to aluminum (Al 6061) for use in spacecraft sampling hardware, both for its potential to be cleaned to sterilization and for its ability to withstand the most effective cleaning protocols.     

THE INFLUENCE OF THE LOCAL INTERSTELLAR MEDIUM ON THE SOLAR WIND DYNAMICS IN THE INNER HELIOSPHERE

The consequences of the interaction between the solar wind and the local interstellar medium for the wind region enclosed by the heliospheric shock are reviewed. After identifying the principal mechanisms to influence the dynamics of the solar wind, an approach allowing the simultaneous incorporation of neutral atoms, pick-up ions, cosmic rays and energetic electrons into a multifluid model of the expanding wind plasma is outlined. The effects of these particle species are discussed in detail, with special emphasis on the electron component which behaves more like a quasi-static hot gas rather than an expanding fluid. This electron gas is effectively trapped within a three-dimensional trough of a circumsolar electric potential whose outer fringes are possibly determined by the density distribution of anomalous cosmic rays. The electrons are proven to be a globally structered component of great importance for the solar wind momentum flow contributing to a triggering of the solar wind dynamics by asymmetric interstellar boundary conditions. Finally, the consequences for the relative motion of the Sun and the local interstellar medium as well as for the solar system as a whole are described.     

Gravimorphogenesis and ultrastructure of the fungus Flammulina velutipes grown in space, on clinostats and under hyper-g conditions.

The D-2-mission provided the facilities to cultivate the higher basidomycete Flammulina velutipes (Agaricales) in space for about 8 days. Gravimorphogenesis of developing fruiting body primordia in weightlessness was documented in comparison to cultures incubated on a 1xg reference centrifuge in space. Chemical fixation of fruiting bodies took place for later ultrastructural analysis. The microgravity grown fruiting bodies exhibited random orientation compared to the 1xg-cultures where fruiting bodies showed exactly negative gravitropic orientation. Weightlessness did not impair fruiting body morphogenesis and growth although flat and helically twisted stipes were observed. Ultrastructural analyses of microgravity-, 1xg- and 20xg-samples did not reveal sedimentable cell components. Gravitropic bending involves growth inhibition at the upper side of a horizontally oriented transition zone, the graviperceptive region of the stipe. The fastest ultrastructural response to the altered direction of the accelerational force is the accumulation of cytosolic vesicles at the lower part of this region. They contribute to the expansion of the central vacuole and therefore to the differential enlargement of the lower side of the stipe.     

Spaceflight hardware allowing unilateral irradiation and chemical fixation in petri dishes.

To accommodate a spaceflight experiment with moss (SPM), experiment-unique equipment (EUE) was developed by engineers at Kennedy Space Center. The hardware allows sterile culture for an extended period of time in commercial petri dishes, lateral illumination of each culture with light of a specific wavelength (660 nm; other wavelengths are possible) and a range of intensities (0.05-5 micromoles photons m-2 s-1), incubation in complete darkness, and chemical fixation to terminate the experiment under conditions of microgravity. The use of a fixative required triple containment to protect the astronaut crew. An external panel on the experiment container allowed the timing of illumination and fixation to be controlled by the crew. Light quality is provided by light emitting diodes (LEDs) that are located in the lid of the outer container, the BRIC (Biological Research In Canisters)-LED. Each canister accommodates 6 Petri Dish Fixation Units (PDFUs), and each PDFU holds one 6 cm petri dish. All components are autoclavable. LED illumination is piped through a transparent glass rod. Each PDFU contains fixative in a reservoir that is released by the depression of an actuator. This hardware performed well during its first flight, the 16-day STS-87 mission in Nov./Dec., 1997 as part of the Collaborative USA and Ukrainian Experiment (CUE). It supported vigorous and sterile moss growth, cells were maintained in position and were well-fixed, and there was a vigorous and consistent response to light. Although here used for moss, in future flight experiments this unique new hardware can be used for many types of organisms normally grown in petri dishes, with or without a requirement for illumination.     

Dosimetric results on EURECA.

Detector packages were exposed on the European Retrievable Carrier (EURECA) as part of the Biostack experiment inside the Exobiology and Radiation Assembly (ERA) and at several locations around EURECA. The packages consist of different plastic nuclear track detectors, nuclear emulsions and thermoluminescence dosimeters (TLDs). Evaluation of these detectors yields data on absorbed dose and particle and linear energy transfer (LET) spectra. Behind a shielding thickness in front of the detectors of 0.09g cm-2 the doses range between 21.26 Gy and 0.87 Gy depending on the location of the dosimeter. Not all measurement can be explained by calculations.     

Vapor hydrogen peroxide as alternative to dry heat microbial reduction

The Jet Propulsion Laboratory (JPL), in conjunction with the NASA Planetary Protection Officer, has selected vapor phase hydrogen peroxide (VHP) sterilization process for continued development as a NASA approved sterilization technique for spacecraft subsystems and systems. The goal was to include this technique, with an appropriate specification, in NASA Procedural Requirements 8020.12 as a low-temperature complementary technique to the dry heat sterilization process.     

Technology challenges for space interferometry: The option of mid-infrared integrated optics

Nulling interferometry is a technique providing high angular resolution which is the core of the space missions Darwin and TPF. The first objective is to reach a deep degree of starlight cancelation in the range 6–20 μm, in order to observe and to characterize the signal from an earth-like planet. Among the numerous technological challenges involved in these missions, the question of the beam combination and wavefront filtering has an important place. A single-mode integrated optics (IO) beam combiner could support both the functions of filtering and the interferometric combination, simplifying the instrumental design. Such a perspective has been explored in this work within the project Integrated Optics for Darwin (IODA), which aims at developing a first IO combiner in the mid-infrared. The solutions reviewed here to manufacture the combiner here are based on infrared dielectric materials on one side, and on metallic conductive waveguides on the other side. With this work, additional inputs are offered to pursue the investigation on mid-infrared photonics devices.     

Estimation of vertically integrated water vapor in Hungary using MODIS imagery

Information about the amount and spatial structure of atmospheric water vapor is essential in understanding meteorology and the Earth environment. Space-borne remote sensing offers a relatively inexpensive method to estimate atmospheric water vapor in the form of integrated water vapor (IWV). The research activity reported in the present paper is based on the data acquired by the HRPT/MODIS (High Resolution Picture Transmission, MODerate resolution Imaging Spectroradiometer) receiving station established in Budapest (Hungary) by the Space Research Group of the Eötvös Loránd University. Integrated water vapor is estimated by the remotely sensed data of the MODIS instrument with different methods and also by the operational numerical weather prediction model of the European Centre for Medium-Range Weather Forecasts (ECMWF). Radiosonde data are used to evaluate the accuracy of the different IWV fields though it has been pointed out that the in situ data also suffers from uncertainties. It was found that both the MODIS and the ECMWF based fields are of good accuracy. The satellite data represent finer scale spatial structures while the ECMWF data have a relatively poor spatial resolution. The high quality IWV fields have proved to be useful for radiative transfer studies such as the atmospheric correction of other satellite data from times different than the overpass times of satellites Terra/Aqua and the forecast times of the model data. For this purpose the temporal variability of IWV is scrutinized both using ECMWF and MODIS data. Taking advantage of Terra and Aqua overpasses, the mean rate of change of IWV estimated by the near infrared method was found to be 0.47 ± 0.45 kg m⁻² h⁻¹, while it was 0.13 ± 0.65 kg m⁻² h⁻¹ based on the infrared method. The numerical weather prediction model’s analysis data estimated −0.01 ± 0.13 kg m⁻² h⁻¹ for the mean growth rate, while using forecast data it was 0.24 ± 0.18 kg m⁻² h⁻¹. MODIS data should be used when available for the estimation of the IWV in other studies. If no satellite data are available, or available data are only from one overpass, ECMWF based IWV can be used. In this case the analysis fields (or the satellite field) should be used for temporal extrapolation but the rate of change should be calculated from the forecast data due to its higher temporal resolution.